Understanding DNA Fragment Separation by Polyacrylamide Gel Electrophoresis

Which o factor (transcription factor) binds to the osmy promoter?

A. Neither
B. 038
C. 070
D. Both

Answer:

In the context of the question, the o factor that binds to the osmy promoter is 070.

The Process of DNA Fragment Separation by Polyacrylamide Gel Electrophoresis

Separating fragments by polyacrylamide gel electrophoresis and visualizing radiolabeled bands on x-ray film is a technique that is used to help in the identification of DNA. When DNA is radiolabeled, this process can be used to separate DNA fragments that have been cut or are uncut, which is essential for further analysis.

Polyacrylamide gel electrophoresis (PAGE) is a technique that uses an electric field to separate DNA fragments according to their size. DNA molecules are negatively charged, which makes them migrate through a polyacrylamide gel matrix when an electric field is applied. Smaller fragments move through the matrix faster and travel further from the point of origin compared to larger fragments. By staining or using a radiolabel to visualize the DNA bands, the fragments can be visualized.

Uncut DNA fragments migrate faster, meaning that they will be close to the wells, while cut fragments migrate slower, and they will be far from the wells. The process can be used to identify cut and uncut fragments, and more analysis can be carried out depending on the nature of the DNA being studied.