Prepare Various Solutions and Determine Dilution Factors
How can we prepare different solutions and calculate dilution factors?
1. Prepare 250 mL of 1.5M ribose solution from solid glucose (MW=150.13 g/mol)
2. Prepare 45ml of 0.15M NaOH from 0.8M NaOH solution
3. Calculate the dilution factor when adding saline solution to serum
4. Find the final dilution after multiple dilution steps
5. Determine the concentration and volume of a final NaCl solution after serial dilutions
Solution Preparation and Dilution Calculation
1. To prepare the ribose solution, calculate the amount of glucose needed.
2. Use the molarity formula to find the volume of initial NaOH solution required.
3. Dilution factor can be determined from the total volume and aliquot volume.
4. Serial dilutions result in a final dilution by multiplying individual dilutions.
5. Final concentration and volume after serial dilutions can be calculated.
Step-by-Step Procedures for Solution Preparation
Preparing the ribose solution involves calculating the amount of glucose needed based on molarity and molecular weight. For the NaOH solution, use the molarity formula to find the volume of the initial solution required. When adding saline solution to serum, the dilution factor can be obtained by dividing the total volume by the aliquot volume. For serial dilutions, multiplying individual dilutions gives the final dilution. Finally, determine the concentration and volume of the final NaCl solution after serial dilutions by calculations.